The basics of cell culture as applied to human cells are discussed. Biosafety when working with human tissue, which is often pathogenic, is important. The requirements for a tissue culture laboratory are described, particularly the range of equipment needed to carry out cell isolation, purification, and culture. Steps must be taken to maintain aseptic conditions to prevent contamination of cultures with micro-organisms.
What is cell culture ?
Cell culture refers to the practice of growing and manipulating cells in vitro, outside their natural environment within a living organism. It is an essential technique in cell biology and biotechnology, allowing the study of cell behavior, understanding biological processes, and developing new therapies and products.
What are the main components of cell culture media?
Cell culture media typically contain a combination of components that provide the necessary nutrients and growth factors for the cells. The exact composition of the media may vary depending on the cell type and specific experimental requirements, but here are some common components found in cell culture media:
Basal Salt Solution: This provides essential inorganic salts, such as sodium, potassium, calcium, and magnesium, which are necessary for cell function and osmotic balance.
Energy Source: Glucose is often included as the primary energy source for the cells.
Amino Acids: Cells require amino acids for protein synthesis. Some cell culture media contain all essential amino acids, while others may require the addition of specific amino acids depending on the cell type.
Vitamins: Essential vitamins, such as vitamins B and C, are included in the media to support cell growth and metabolism.
Growth Factors: Certain cell types may require specific growth factors, such as epidermal growth factor (EGF), fibroblast growth factor (FGF), or insulin-like growth factor (IGF), to stimulate their growth and proliferation.
Serum: Fetal bovine serum (FBS) or other types of animal serum are often added to provide additional nutrients, growth factors, and hormones that support cell growth and attachment.
Buffers: Buffers, such as HEPES or bicarbonate, help maintain the pH of the media within a physiological range.
Antibiotics/Antimycotics: These are often added to prevent bacterial or fungal contamination in the culture.
MycoBlue Mycoplasma Detector : rapid detection of mycoplasma contamination in cell culture:
Its advantage is convenience, after adding 1 μl of the cell culture supernatant to the reaction system and incubating at 60℃ for 1 h, the results can be determined by visual observation. Detection can be easily completed in cell culture lab without performing PCR, qPCR, or electrophoresis.
Compared with conventional PCR methods, MycoBlue Mycoplasma Detector is more resistant to the inhibitor in the culture supernatant, avoiding weak positive and false negative. There is no need to perform electrophoresis, avoiding false positive from aerosol contamination.
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Annexin V Apoptosis Detection Kits:
Apoptosis, the most studied type of programmed cell death, is an active, highly ordered process involving a series of enzymes. Apoptosis plays a key role in ensuring the health of multicellular organisms and in the normal development of individuals. In recent years, apoptosis has become one of the hottest topics in the field of biology.